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Connectivity issues with SQL server after updating clients from W7 to W10

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We are having problems with our database since we moved clients over from Windows 7 to Windows 10 last year.

This is the current situation:

Windows 7, Sophos AV: applications are working fine with our database.
Windows 10 1903,1909, Sophos AV: DB Connection breaks down once a day, some minutes after login, on ALL Windows 10 PCs , no matter which manufacturer (Fujitsu, Lenoco..). After starting applications again it will work mostly for the rest of the day.

The database server is a Windows Server 2012R2 with SQL 2016 fully patched.

What we tried so far:

  • Energy saving options to High Performance
  • Disable Energy Saving for Networkcards
  • Set GPO to wait for network connection on boot
  • Set ClientPCs to prefer IPV4 before IPV6
  • Latest firmware on all Switches
  • Installed Windows 10 from scratch on a clients which had worked before with Windows 7...

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